Isolation of Mesophyll Protoplasts from Prunus domestica cv. Quetche
Institute for Plant Protection, BG – 2230 Kostinbrod, Bulgaria
KONDAKOVA, V., 1999. Isolation of mesophyll protoplasts from Prunus domestica cv. Quetche. Bulg. J. Agric. Sci., 5: 411-414
An effective system for isolation of mesophyll protoplasts was developed. The effect of several enzyme mixtures was tested. An yield of 20.4 x106 protoplsts/g leaf tissue with viability 92% was obtained after digestion of tissue in enzyme solution containing cellulase "Onozuca" RS – 1%, hemicellulase – 1%, pectolyase Y-23 – 0.1% and 0.7M manitol as osmotic.
The plating of protoplsts in medium containing MS basal medium with NAA – 0.01 mg/l, BAP – 1.5 mg/l and zeatine – 0.5 mg/l with osmotic as 0.5M sucrose had better influence on the beginning of first division and development of protoplasts.
Key words: Prunus domestica, mesophyll protoplasts, enzymes, osmotics