Arsenic Accumulation and Effect on Peroxidase Activity in Green Bean and Tomatoes
E. MITEVA1 and S. PEYCHEVA2
1 Plant Protection Institute, Department of Radiobiology and Tehnogenic Pollution, BG – 2230 Kostinbrod, Bulgaria
2 Sofia University "St. Climent Ohridski", Faculty of Biology, Department of Plant Physiology, BG – I42I Sofia, Bulgaria
MITEVA, E. and S. PEYCHEVA, 1999. Arsenic accumulation and effect on peroxidase activity in green bean and tomatoes. Bulg. J. Agric. Sci., 5: 737-740
The accumulation of arsenic in leaves of green bean (Phaseolus vulgaris) and tomatoes (Licopersicum esculentum) and his effect on peroxidase (PO) activity were investigated. The experiments were carried out in green house conditions. The plants were grown in pots, containing I kg soil, contaminated with As in soluble form and concentration 50 mg.kg -1. The plants were harvested after forming of 4th leaf. The As content and PO activity were established in the Ist, 2nd, 3rd and 4th leaf in two plants. The results show that the green bean plants accumulate As more intensively than tomato plants. The accumulated As in plant tissue lead to a response of plants and it's reflected by an increase in the 2nd leaf for two plant species. The response of green bean plants is rather different from this of tomato plants. The involved element induces PO synthesis in all four phases of development of green bean plants, while the involved As in tomatoes causes an induction of PO synthesis only in the Ist and 2nd leaf.
Key words: arsenic, arsenic toxicity, heavy metal stress, peroxidase activity