Effect of the Synthetic Pyrethroid Deltamethrin on the Level of Sulfhydryl Groups in Rat Liver


1Institute of Animal Science, BG-2232 Kostinbrod, Bulgaria
2Institute of Biology and Immunology of Reproduction, BAS, BG-1113 Sofia, Bulgaria


CHESHMEDJIEVA, S., P. POPOVA, T. MODEVA, I. DIMITROVA and Ya.PROFIROV, 1999. Effect of the synthetic pyrethroid deltamethrin on the level of sulfhydryl groups in rat liver. Bulg.J.Agric.Sci., 5: 511-514

The concentrations of total sulfhydryls (TSH), non-protein-bound sulfhydryls (NP-SH) and protein-bound sulfhydryls (PB-SH), as well as the activity glutathione S-transferase [EC] (GST) were determined in the liver of male Wistar rats, treated with 1/1000 and 1/100 of LD50 of the synthetic pyrethtoid deltamethrin [NRDC-161: (S)-a-cyano-m-phenoxybenzyl (1R, 3R)-3-(2,2-dibromovinyl)-2,2-dimethyl-cyclopropane-carboxilate] for ten days per os. It was established a significant decrease in the concentration of low-molecular thiols as a result of the treatmens - about 50 %, with 1/1000 of LD50 of deltamethrin (P < 0.01) and about 63 %, with 1/100 of LD50 of deltamethrin (P < 0.001). As a result of the treatment a considerable reduction in the specific cytosolic activity of GST, measured with o-dinitrobenzene as substrate, was observed. The alterations in thiol/disulfide ratio and glutathione S-transferase activity might play important roles in the toxicity and the detoxifying mechanism of the liver.
Key words: rat liver, total sulfhydryls, non-protein-bound sulfhydryls, protein-bound sulfhydryls, glutathione S-transferase, deltamethrin [NRDC-161: (S)-a-cyano-m-phenoxybenzyl (1R, 3R)-3-(2,2-dibromovinyl)-2,2-dimethyl-cyclopropane-carboxilate]
Abbreviations: TSH - total sulfhydryls; NP-SH - non-protein-bound sulfhydryls; PB-SH - protein bound sulfhydryls; GST - glutathione S-transferase; GSH - glutathione; GSSG - oxidized form of glutathione; G6PDH - glucose-6-phosphate dehydrogenase; GR - glutathione reductase