A Light Microscopic Study on the Alpha Naphthyl Acetate Esterase Activity of the Peripheral T Lymphocytes in Brown Bears (Ursus arctos)

M. BALIKCIER1 and R. N. ASTI2

1 University of Uludag, Faculty of Veterinary Medicine, Department of Histology and Embriology, 16059, Bursa, Turkey mine@uludag.edu.tr
2 University of Ankara, Faculty of Veterinary Medicine, Department of Histology and Embriology, 06110, Ankara, Turkey astư@veterinary.ankara.edu.tr

Abstract

BALIKCIER, M. and R. N. ASTI, 2000. A light microscopic study on the alpha naphthyl acetate esterase activity of the peripheral T lymphocytes in brown bears (Ursus arctos). Bulg. J. Agric. Sci., 6: 195-199

In this study peripheral blood T lymphocytes of brown bears (Ursus arctos) were determined by using a cytochemical staining technique.
Alpha naphtyl acetate esterase (ANAE) staining revealed that large majority of the peripheral blood lymphocytes (87%) were positive, whereas smaller proportion (13%) were negative. Positive lymphocytes showed two distinct granular positivity. Most of the ANAE positive cells (84% of the total) had 1-4 large dot-like reddish brown granules (localised granular positivity). A small quantity of the positive cells (3% of the total) had diffusely arranged small granules (diffuse granular positivity).
Eosinofilic granulocytes and monocytes also showed a diffuse granular positivity.

Key words: ANAE activity, T-lymphocytes, bear