Identification and Characterization of Cucumber Mosaic Virus (CMV) from Bean (Phaseolus vulgaris L.) in Bulgaria

S. TSORLIANIS¹, D. KOSTOVA² and N. J. SPENCE³

¹Agricultural University, BG-4000 Plovdiv, Bulgaria
²Maritsa Vegetable Crops Research Institute, BG-4003 Plovdiv, Bulgaria
³Horticulture Research International, Wellesbourne, Warwick CV35 9EF, UK

Abstract

TSORLIANIS, S., D. KOSTOVA and N. J. SPENCE, 2000. Identification and characterization of Cucumber mosaic virus (CMV) from bean (Phaseolus vulgaris L.) in Bulgaria. Bulg. J. Agric. Sci., 6: 147-154

CMV isolates from Phaseolus vulgaris in Bulgaria were identified and characterized using various polyclonal (PAbs) and monoclonal (MAbs) antibodies in ELISA as well as PCR. CMV isolates were classified as subgroup I. MspI enzyme digestion of PCR amplicons revealed in some isolates patterns similar to subgroup Ig.

Key words: bean, CMV, subgroups, ELISA, PCR, Phaseolus vulgaris