Effect of Formaldehyde on the Growth and Protein Biosynthesis of Candida didensii 74-10 in Transient Regimes under Nitrogen Limitation

B. I. TZVETKOVA*, Ch. S. KATRANUSHKOVA, L. K. LOSSEVA and Ts. A. HRISTOZOVA

Bulgarian Academy of Sciences, Institute of Microbiology, BG-1113 Sofia, Bulgaria
* Institute of Microbiology, Bulgarian Academy of Sciences, Akad. G. Bonchev str., bl. 26 Sofia 1113, Bulgaria, Fax: +359-2-700109, E-mail: Ivanova @ bas.bg

Abstract

TZVETKOVA, B. I., Ch. S. KATRANUSHKOVA, L. K. LOSSEVA and Ts. A. HRISTOZOVA, 2000. Effect of formaldehyde on the growth and protein biosynthesis of Candida didensii 74-10 in transient regimes under nitrogen limitation. Bulg. J. Agric. Sci., 6: 339-344

We carried out our investigation on stain C.didensii 74-10 under contiuous cultivation, nitrogen limitation and dilution rate of 0.1 h–1 after pulse on the yeast populations with 0.01%, 0.02% or 0.04% formaldehyde. The results showed that during the transient regime after pulse with 0.02% formaldehyde it was observed changes in biomass and protein content. At a new steady state the values of the main parameters reached those of the control culture without inhibitor. The data showed an increase of activities of NADPH– and NADH-glutamate dehydrogenase. It was found that under nitrogen limitation after pulse with 0.04% formaldehyde the cell growth was extremely inhibited, biomass and rpotein yields were sharply decreased, and the culture could not rech a new steady state, because of unability to get over the inhibiting impact of the formaldehyde.

Key words: formaldehyde, growth, Candida diddensii, nitrogen limitation, transient regimes