Possibilities for Establishing an Elite Mother Plant Stand of Promising Grapevine Cultivars and Clones in Bulgaria Through the Method of Tissue Culture

1 Agricultural University*, Department of Viticulture, BG-4000 Plovdiv, Bulgaria
2 Experiment Station of Viticulture, BG-4490 Septemvri, Bulgaria


PANDELIEV, S., I. TZVETKOV and V. ROYTCHEV, 2001. Possibilities for establishing an elite mother plant stand of promising grapevine cultivars and clones in Bulgaria through the method of tissue culture. Bulg. J. Agric. Sci., 7: 23-28

In the period 1986-1988, complex investigations were carried out on the growth and generative behaviours of grapevine plants, produced in vitro from a large number of cultivars and rootstocks. It was established a balanced nutrient medium with some modifications for dessert and wine cultivars and rootstocks, on which a high propagation coefficient of 10-5 and good uniformity and plant viability were achieved. An original substrate, composed of waste agricultural materials – flax shavings (50%), peat (20%), rice husks (20%), cattle manure (5%) and river sand (5%) with very good agrophysical and agrochemical properties, well balanced with nutrient macro– and microelements, was created. No genetic deviations were observed to the second passage of the micropropagated plants. Only some differences of epigenetic nature were found in the first years, which later disappeared. The grapevines obtained from tissue culture were distinguished for their vigorous growth and high biological productivity. No significant differences were established between them and controls in terms of yield and quality of wines produced. The propagation through the tissue culture technique is a promising method which could successfully be used for rapid establishment of an elite mother plant stand for the production of grafted and rooted grapevines in Bulgaria.

Key words: grapevine, tissue culture, substrate, propagation, fertility, yield, grape, quality