Regeneration of Plants from Initial Calluses of Wood Betony (Stachys officinalis (L.) Trev.)

1 University of Shumen "Episcop Konstantin Preslavsky", BG-9700 Shumen, Bulgaria
2 Agricultural Institute, BG-9700 Shumen, Bulgaria


ZAHARIEV, D. and Y. SLAVOVA, 2004. Regeneration of plants from initial calluses of Wood Betony (Stachys officinalis (L.) Trev.). Bulg. J. Agric. Sci., 10: 573-577

The focus of our research is to investigate growth media, in which abundant callus formation and partial direct organ formation have been achieved from various explants of Stachys officinalis (L.) Trev. We succeeded in regenerating plants from initial calluses, which have been formed in plants' base while they were multiplied in vitro. In growth medium, where 0.2 mg/l BAP (6-benzilaminopurine) participates, we achieved an indirect formation of organs from leaf blades (18.8%) and leaf stalks (5.4%).

Key words: Stachys officinalis (L.) Trev., regeneration of plants, growth medium, callus, callus formation, conditions in vitro, root, leaf stalk, leaf blade