The Carbohydrates in the Culture Medium Effect Metabolism of Cherry (Prunus avium L.) Callus

Fruit Growing Institute, BG - 4004 Plovdiv, Bulgaria


GERCHEVA, P., P. SHALAMANOVA and P. MANOLOV, 2005. The carbohydrates in the culture medium effect metabolism of cherry (Prunus avium L.) callus. Bulg. J. Agric. Sci., 11: 273-280

Cultural media with different types and concentrations of carbohydrates have been tested intensively during the last few years in order to improve the morphogenetic response of some recalcitrant species in vitro.
The aim of the present study was to investigate the effect of carbohydrates on the metabolism of sweet cherry (Prunus avium L.) callus in vitro.
The experiments have been carried out with cherry callus lines of leaf origin. To study metabolic response of the callus, 14C labelled sucrose alone and in combinations with non-labelled sorbitol and manitol were used. The 14C dynamics in the main organic fractions and compounds in the process of 14C-sucrose metabolism were investigated.
The results confirmed that the manitol could not be utilized by cherry tissues and thus it could be used as an osmotic control in the experiments. In the view of data from biochemical experiments, we can consider that sorbitol has a metabolic (not osmotic) effect. The mechanisms of its uptake and methabolism seem to be specific and to differ from those of sucrose.

Key words: 14C, sucrose, sorbitol, callus, in vitro, sweet cherry