Radiobiological Studies on the Greater Wax Moth, Galleria mellonella L. (Lepidoptera: Pyralidae).
II. Radiation Induced Sterility

Plant Protection Institute, BG-2230 Kostinbrod, Bulgaria


MILCHEVA, R.Y., 2005. Radiobiological studies on the Greater Wax Moth, Galleria mellonella L. (Lepidoptera: Pyralidae). II. Radiation induced sterility. Bulg. J. Agric. Sci., 11: 423-430

Results of studies on the radiation induced sterility after gamma irradiation of mature pupae and imago of the greater wax moth are reported. Sterility was determined on the basis of non-hatched eggs.
After irradiation of mature pupae the sterilizing doses for females and males are 200 and 250 Gy respectively. The cross of two irradiated parents results in sterility at 150 Gy. The estimated SD50 and SD100 are 152 and 358 Gy for male pupae and 77 and 333 Gy for female pupae respectively. There is a slight difference in the radioresistance of the two sexes, when they are irradiated as pupae.
The main cause for radiation induced sterility in GWM is the induction of dominant lethal mutations in gametes. Dominant lethals are manifested not only in embryonic, but also in larval stage.
Adults are more radiosensitive than mature pupae (sterilizing doses 77 Gy and 243 Gy for females and males respectively). Compared to males, female moths are more sensitive to radiation. Male moths are more radiosensitive than the mature male pupae, but the difference is not as considerable as in females.
Female fecundity decreases at doses 350 Gy. Compared to the control, female longevity is increased after irradiation, while males longevity decreases.

Key words: Galleria mellonella, gamma radiation, radiation induced sterility, dominant lethal mutations
Abbreviations: GWM-greater wax moth; SIT - Sterile Insect Technique; SD - sterilizing dose; IM - irradiated male; IF - irradiated female; NM - non-irradiated male; NF - non-irradiated female