Rapid Propagation of the Medicinal Plant Pinellia ternata by in vitro Leaves Culture

Z. S. PENG, C. K. LUO, P. CAI, Z. C. MAO, C. Y. KANG and J. YANG
College of Life Science, China West Normal University, Nanchong, Sichuan, 637002, P.R.China


PENG, Z. S., C. K. LUO, P. CAI, Z. C. MAO, C. Y. KANG and J. YANG, 2007. Rapid propagation of the medicinal plant Pinellia ternata by in vitro leaves culture. Bulg. J. Agric. Sci., 12: 01-06

Pinellia ternata (Thunb) Breit is an important medicinal plants. Its tubers,being called as Banxia in Chinese words, were a crude drug of traditional Chinese medicine and some pharmaceutical products. For satisfying the demand of the drug market, some Chinese farmers began to cultivate this species in the field, and some breeders are work hard to domesticate this species into a crop. Dislike that of normal crops, the propagation rate of P. ternata is very low in nature condition. This study introduced a rapid propagation method by direct organogenesis of Pinellia ternata plantlets. This method used Pinellia ternata leaf as explants, and the regenerated in vitro plantlets obtained directly on the MS medium supplemented with the 0.5 mg/l benzyladenine and 0.5 mg/l α-naphthalene acetic acid. After transplanted into soil, the in vitro plantlets themselves showed rapid growing and high nature propagation rate. In addition, the tubers of in vitro plants much less polluted by heavy mental such as plumbum, cadmium, hydrargyrum and arsenic, after a session growing in soil.

Key words: Pinellia ternata, in vitro plantlet, medicinal plant, rapid propagation

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