Z. NANOVA1, Y. SLAVOVA2, D. NENKOVA3 and I. IVANOVA3
1 Konstantin Preslavsky University of Shumen, BG - 9700 Shumen, Bulgaria
2 Agricultural Institute, BG - 9700 Shumen, Bulgaria
3 Institute of Cryobiology and Food Technologies, BG - 1504 Sofia, Bulgaria
NANOVA, Zh., Y. SLAVOVA, D. NENKOVA and I. IVANOVA, 2007. Microclonal propagation of hyssop (Hyssopus officinalis L.). Bulg. J. Agric. Sci., 13: 213-219
A successful trial for tissue culture introduction and micropropagation of the medicinal plant hyssop (Hyssopus officinalis L.) has been conducted. Stem tips from plants vigorously growing under controlled conditions were used as initial explants. The introduction and micropropagation were performed in nutritive media with participation of biologically active substances (BAS) as BI (Benzimidazol) - 0.2 or 0.5 mg/l and PP - 40 (derivative of the phenoxy acetic acid and the folic acid) - 0.5 mg/l. On a nutritive medium containing 0.1 mg/l of IAA (indolyl acetic acid) and 0.1 mg/l of BI, rooting percentage of 90.0% ± 3.0% and normal growth of plants with medium sized root and vegetative parts have been achieved.