Thidiazuron-induced Regeneration in Root Segments of White Poplar (P. alba L.)

I. TSVETKOV¹, J-F. HAUSMAN² and L. JOUVE^2
1Forest Research Institute, BG - 1756 Sofia, Bulgaria
²CRP Gabriel Lippmann, CREBS, 41, Department of Environment and Agrobiotechnology, Rue du Brill, L- 422 BELVAUX, Luxembour

Abstract

TSVETKOV I., J-F. HAUSMAN and L. JOUVE, 2007. Thidiazuron-induced regeneration in root segments of white poplar (P. alba L.). Bulg. J. Agric. Sci., 13: 623-626

Root segments isolated from stably proliferating in vitro clone of white poplar (P. alba L.) were cultivated on modified MS medium supplemented with different thidiazuron concentrations. The thidiazuron concentration significantly affected the regenerative capacity of the explants. The lower doses firmly stimulated the regenerative ability of the root segments, while the stronger ones demonstrated inhibitive organogenic effect. The highest number of regenerated shoots was formed on medium supplemented with 0.56 µM thidiazuron, but longest shoots were produced at 0.02 µM thidiazuron.

Key words: root explants, in vitro regeneration, thidiazuron, Populus spp.
Abbreviations: IAA – indole-3-acetic acid, BAP – 6-benzylaminopurine, MS – Murashige & Skoog nitrient medium, TDZ (thidiazuron) – 1-Phenyl-3-(1,2,3-thiadiasol-5-yl)urea

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