O. TASINOV, Y. KISELOVA-KANEVA and D. IVANOVA
Medical University “Prof. Dr. P. Stoyanov”, Department of Biochemistry, Molecular Medicine and Nutrigenomics, BG – 9002 Varna, Bulgaria
Tasinov, O., Y. Kiselova-Kaneva and D. Ivanova, 2013.Sambucus ebulus L. fruit aqueous infusion modulates GCL and GPx4 gene expression. Bulg. J. Agric. Sci., Supplement 2, 19: 143–146
Glutamate-cysteine ligase and glutathione peroxidase 4 are enzymes involved in cellular antioxidant defense. Biologically active compounds, such as polyphenols, may modulate their gene expression. Sambucus ebulus L. is a plant, which fruit infusions exhibits high antioxidant activity, due to its high polyphenol content. We analyzed the effect of Sambucus ebulus fruit aqueous infusion on glutamate-cysteine ligase catalytic subunit and glutathione peroxidase 4 gene expression levels in the presence and in the absence of oxidizing agent tert-butyl hydroperoxide in a 3T3-L1 preadipocyte cell culture model. Gene expression was analyzed by Real-Time qPCR, and relative gene expression levels were calculated using 2–ΔΔCt method. Viability of treated preadipocytes was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromid reduction assay. In the range of 1.25% to 12.5% content of the infusion in the culture medium cell viability was not affected significantly. Glutamate-cysteine ligase mRNA levels were increased significantly (p < 0.05) after treatment. Pretreatment decreased tert-butyl hydroperoxide-induced gene expression of glutamate-cysteine ligase (p < 0.05) and of glutathione peroxidase 4 (p < 0.05). These data suggest that Sambucus fruit infusion exhibits strong antioxidant activity by modulating glutamate-cysteine ligase and glutathione peroxidase 4 gene expression. The study provides first scientific data about the antioxidant activity of Sambucus ebulus fruit infusion in a cell culture model.