Agrimonia Eupatoria L. Extract Modulates Glutamate-Cysteine Ligase and Glutathione Peroxidase Expression in 3T3-L1 Cells

Medical University, BG – 9000 Varna, Bulgaria


Ivanova, D., O. Tasinov, D. Vankova and Y. Kiselova-Kaneva, 2013. Agrimonia eupatoria L. extract modulates glutamate-cysteine ligase and glutathione peroxidase expression in 3T3-L1 cells. Bulg. J. Agric. Sci., Supplement 2, 19: 171–174


Oxidative stress is implicated in a number of diseases and pathologic conditions. Expression of antioxidant enzymes may be influenced by a variety of substances, including antioxidants and plant polyphenols. The aim of the study was to assess the expression of glutamate-cysteine ligase and glutathione peroxidase in cell culture model upon treatment with Agrimonia eupatoria extract in standard conditions and in oxidatively stimulated cells. Incubation of 3T3-L1 cells with the extract resulted in significant increase in mRNA levels of glutamate-cysteine ligase. No significant change in transcriptional level of glutathione peroxidase was detected. Tert-butyl-hydroperoxide significantly upregulated expression of both enzymes. Pretreatment of cells with extract significantly reduced the stimulatory effect of the oxidizing agent on gene expression.

Key words: Agrimonia eupatoria, gene expression, GCL, GPx, 3T3-L1
Abbreviations: AE –Agrimonia eupatoria extract; t-BH - Tert-butyl-hydroperoxide; GCL – glutamate-cysteine ligase; GPx4 - glutathione peroxidase, GSH - reduced glutathione

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