Antimicrobial Susceptibility of Staphylococci Isolated from Clinical Mastitis in Dairy Cows in Kosovo

University of Prishtina, Faculty of Agriculture and Veterinary, Str. Bill Clinton, n.n., 10000, KOSOVO


HAMIDI, A. and D. SYLEJMANI, 2016. Antimicrobial susceptibility of staphylococci isolated from clinical mastitis in dairy cows in Kosovo. Bulg. J. Agric. Sci., 22: 836–839

This study was carried out to evaluate antibiotic susceptibility of 26 strains of coagulase negative staphylococci (CNS) and 18 strains of Staphylococcus aureus isolated from samples of dairy cows with clinical mastitis. The isolates species of staphylococci were identifi ed using coagulase test (using rabbit plasma) and API Staph system (bioMėrieux SA) which differentiates S. aurues from other staphylococci. Isolates were tested for antimicrobial susceptibility on Mueller Hinton agar by dick diffusion method according to the Clinical Laboratory Standards Institute. The fi ndings of this study showed that the most effective in vitro antibiotics for S. aureus isolates, isolated from cows with clinical mastitis wereamoxycillin/clavulanic acid (83.3%), tetracycline (77.8%), gentamycin (77.8%) and trimethoprim (66.7%) and for CNS isolates trimethoprim (88.46%), amoxycillin (84.6%), tetracycline (77%) and gentamycin (69.2%) while the isolates of S. aureus and CNS were found to be more resistant to penicillin (55.5% and 53.8%, respectively), streptomycin (50% and 46.1%, respectively) and ampicillin (38.9% and 46.1%, respectively). Antimicrobial susceptibility tests are important for the selection of the most effective antimicrobial agent for treatment of bovine mastitis caused by staphylococci (Staph. aureus and CNS).Continous monitoring for antimicrobial susceptibility of Staphylococci isolated from dairy cows with clinical mastitis is recommended as an important component of effi ciently and prudent antimicrobial use practices.

Key words: Antimicrobial susceptibility/ staphylococci/ bovine mastitis/ Kosovo.

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